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# desert_storm # [userpic]

has anyone else noticed this 'error' in X Files?

December 1st, 2008 (03:59 pm)
frustrated

Mood: frustrated

'Use of PCR to compare DNA'

For prime example, in Christmas Carol (season 5).
Scully orders a PCR (polymerase chain reaction) to compare Emily Sim's DNA with that of her sister Melissa.
God I wanna scream at the screen.

A PCR only replicates teh DNA into substantial amounts, one small strand suddenly now has a million copies in a very short time. The actual procedure Scully uses to compare the DNA is to perform a gel electrophoresis to create the karyotype (the transparent sheets with the black lines on to represent the chromosomes). The size of the black lines are indicative of the amounts of DNA used in the original sample.



Man, I guess paying attention in Biology is a good thing, cos I understand a hell of a lot more of what is going on, medically-wise, but I also see the small errors as well....oh so slightly infurating when the same thing pops up again and again.


/rant

(any other errors that anyone has noticed? Please comment them if so...thankies!)


We have also found the wrong number for Napier's Constant was used, and also Scully writes ademine instead of adenine for the nucleotide bases translated during The Sixth Extinction

Comments

Posted by: nodazzle ([info]nodazzle)
Posted at: December 1st, 2008 05:06 am (UTC)

that's irritating.

i'm pretty sure they also got napier's constant wrong in paper clip. i can't remember the exact error... i just remember watching the episode WHILE doing calculus and working with napier's constant and then hearing scully give the wrong number.

doods. really. it's a constant. look it up.

:)

- karen

eta - ya... i looked it up... napier's constant is 2.71828 and they use the number 2.7828

Edited at 2008-12-01 05:10 am (UTC)

Posted by: # desert_storm # ([info]desert_storm494)
Posted at: December 15th, 2008 10:28 pm (UTC)
scully // gun

I found another one :)

In The Sixth Extinction I, Scully is translating the Navajo symbols from the craft's surface, and she writes down the four nucleotide bases, only she spells one wrong lol. She writes cytosine (right), thymine (right), guanine (right) and ademine (wrong!). It is adenine with an 'n'.

hehe lol

Posted by: xtoothfairy ([info]xtoothfairy)
Posted at: December 1st, 2008 12:47 pm (UTC)

You are so right! I never really payed attention to it, but you're right. PCR is not the way to determine the karyotype. I'm bummed now that I didn't figure that out myself.
I've forgotten about the exact workings of gel electrophoresis, so I'll take your word on that.

Posted by: # desert_storm # ([info]desert_storm494)
Posted at: December 1st, 2008 07:46 pm (UTC)

restriction enzymes cut the DNA into fragments, a positive plate is placed at the other end of the gel, while a negative plate is at the end with the DNA. DNA is negatively charged and so is drawn through the gel to the positive plate. The fragments are different sizes and so drawn through at different rates. The DNA is generally binded with luminescing markers to enable to see it clearly.

Posted by: xtoothfairy ([info]xtoothfairy)
Posted at: December 1st, 2008 07:49 pm (UTC)

Thanks for re-teaching me! :)

Posted by: # desert_storm # ([info]desert_storm494)
Posted at: December 15th, 2008 10:27 pm (UTC)
scully // dear lj

I found another one :)

In The Sixth Extinction I, Scully is translating the Navajo symbols from the craft's surface, and she writes down the four nucleotide bases, only she spells one wrong lol. She writes cytosine (right), thymine (right), guanine (right) and ademine (wrong!). It is adenine with an 'n'.

hehe lol